human genome wide library Search Results


human crispri dual sgrna libraries  (Addgene inc)
95
Addgene inc human crispri dual sgrna libraries
( A ) Comparison of growth phenotypes for all elements between our pilot single-sgRNA library and Horlbeck et al. data, merged by gene name (n=20,228 elements). Growth phenotypes are reported as γ (log 2 fold-enrichment of T final over T 0 , per doubling) and correlated between experiments (r=0.82). ( B ) Comparison of growth phenotypes for all elements between our pilot dual-sgRNA library and Horlbeck et al. data, merged by gene name (n=20,228 elements). Growth phenotypes are reported as γ and correlated between experiments (r=0.83). ( C ) Comparison of growth phenotypes for all elements between our pilot single- and dual-sgRNA libraries, merged by gene name (n=21,239 with 20,228 targeting elements and 1011 non-targeting elements). Growth phenotypes are reported as γ and correlated between experiments (r=0.86). ( D ) Comparison of true and false-positive rates in single element screens. ‘Positives’ (n=1363 elements) were defined as genes with a K562 <t>CRISPRi</t> growth screen p-value <0.001 and γ<–0.05 , and ‘negatives’ were defined as non-targeting control sgRNA pairs (n=1011 elements). ( E ) Comparison of recombination rates for non-targeting dual-sgRNA elements between replicates of our K562 growth screen. Non-targeting elements with a growth phenotype (γ>0.05 or γ<−0.05) were excluded (n=973 elements). Recombination rates were weakly correlated between replicates (r=0.30). ( f ) Comparison of recombination rates for all dual-sgRNA elements between replicates of our K562 growth screen (n=20,387 elements). Recombination rates were strongly correlated between replicates (r=0.77). ( G ) Comparison of recombination rates and growth phenotypes for all dual-sgRNA elements in our K562 growth screen (n=20,387 elements). Growth phenotypes are reported as γ. Recombination rates were strongly anticorrelated with growth phenotypes (r=−0.84).
Human Crispri Dual Sgrna Libraries, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human crispri dual sgrna libraries/product/Addgene inc
Average 95 stars, based on 1 article reviews
human crispri dual sgrna libraries - by Bioz Stars, 2026-06
95/100 stars
  Buy from Supplier
gene  (Addgene inc)
94
Addgene inc gene
( A ) Comparison of growth phenotypes for all elements between our pilot single-sgRNA library and Horlbeck et al. data, merged by gene name (n=20,228 elements). Growth phenotypes are reported as γ (log 2 fold-enrichment of T final over T 0 , per doubling) and correlated between experiments (r=0.82). ( B ) Comparison of growth phenotypes for all elements between our pilot dual-sgRNA library and Horlbeck et al. data, merged by gene name (n=20,228 elements). Growth phenotypes are reported as γ and correlated between experiments (r=0.83). ( C ) Comparison of growth phenotypes for all elements between our pilot single- and dual-sgRNA libraries, merged by gene name (n=21,239 with 20,228 targeting elements and 1011 non-targeting elements). Growth phenotypes are reported as γ and correlated between experiments (r=0.86). ( D ) Comparison of true and false-positive rates in single element screens. ‘Positives’ (n=1363 elements) were defined as genes with a K562 <t>CRISPRi</t> growth screen p-value <0.001 and γ<–0.05 , and ‘negatives’ were defined as non-targeting control sgRNA pairs (n=1011 elements). ( E ) Comparison of recombination rates for non-targeting dual-sgRNA elements between replicates of our K562 growth screen. Non-targeting elements with a growth phenotype (γ>0.05 or γ<−0.05) were excluded (n=973 elements). Recombination rates were weakly correlated between replicates (r=0.30). ( f ) Comparison of recombination rates for all dual-sgRNA elements between replicates of our K562 growth screen (n=20,387 elements). Recombination rates were strongly correlated between replicates (r=0.77). ( G ) Comparison of recombination rates and growth phenotypes for all dual-sgRNA elements in our K562 growth screen (n=20,387 elements). Growth phenotypes are reported as γ. Recombination rates were strongly anticorrelated with growth phenotypes (r=−0.84).
Gene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gene/product/Addgene inc
Average 94 stars, based on 1 article reviews
gene - by Bioz Stars, 2026-06
94/100 stars
  Buy from Supplier
human improved genome wide knockout crispr library v1  (Addgene inc)
95
Addgene inc human improved genome wide knockout crispr library v1
( A ) Comparison of growth phenotypes for all elements between our pilot single-sgRNA library and Horlbeck et al. data, merged by gene name (n=20,228 elements). Growth phenotypes are reported as γ (log 2 fold-enrichment of T final over T 0 , per doubling) and correlated between experiments (r=0.82). ( B ) Comparison of growth phenotypes for all elements between our pilot dual-sgRNA library and Horlbeck et al. data, merged by gene name (n=20,228 elements). Growth phenotypes are reported as γ and correlated between experiments (r=0.83). ( C ) Comparison of growth phenotypes for all elements between our pilot single- and dual-sgRNA libraries, merged by gene name (n=21,239 with 20,228 targeting elements and 1011 non-targeting elements). Growth phenotypes are reported as γ and correlated between experiments (r=0.86). ( D ) Comparison of true and false-positive rates in single element screens. ‘Positives’ (n=1363 elements) were defined as genes with a K562 <t>CRISPRi</t> growth screen p-value <0.001 and γ<–0.05 , and ‘negatives’ were defined as non-targeting control sgRNA pairs (n=1011 elements). ( E ) Comparison of recombination rates for non-targeting dual-sgRNA elements between replicates of our K562 growth screen. Non-targeting elements with a growth phenotype (γ>0.05 or γ<−0.05) were excluded (n=973 elements). Recombination rates were weakly correlated between replicates (r=0.30). ( f ) Comparison of recombination rates for all dual-sgRNA elements between replicates of our K562 growth screen (n=20,387 elements). Recombination rates were strongly correlated between replicates (r=0.77). ( G ) Comparison of recombination rates and growth phenotypes for all dual-sgRNA elements in our K562 growth screen (n=20,387 elements). Growth phenotypes are reported as γ. Recombination rates were strongly anticorrelated with growth phenotypes (r=−0.84).
Human Improved Genome Wide Knockout Crispr Library V1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human improved genome wide knockout crispr library v1/product/Addgene inc
Average 95 stars, based on 1 article reviews
human improved genome wide knockout crispr library v1 - by Bioz Stars, 2026-06
95/100 stars
  Buy from Supplier
human genome wide library  (Addgene inc)
94
Addgene inc human genome wide library
( A ) Comparison of growth phenotypes for all elements between our pilot single-sgRNA library and Horlbeck et al. data, merged by gene name (n=20,228 elements). Growth phenotypes are reported as γ (log 2 fold-enrichment of T final over T 0 , per doubling) and correlated between experiments (r=0.82). ( B ) Comparison of growth phenotypes for all elements between our pilot dual-sgRNA library and Horlbeck et al. data, merged by gene name (n=20,228 elements). Growth phenotypes are reported as γ and correlated between experiments (r=0.83). ( C ) Comparison of growth phenotypes for all elements between our pilot single- and dual-sgRNA libraries, merged by gene name (n=21,239 with 20,228 targeting elements and 1011 non-targeting elements). Growth phenotypes are reported as γ and correlated between experiments (r=0.86). ( D ) Comparison of true and false-positive rates in single element screens. ‘Positives’ (n=1363 elements) were defined as genes with a K562 <t>CRISPRi</t> growth screen p-value <0.001 and γ<–0.05 , and ‘negatives’ were defined as non-targeting control sgRNA pairs (n=1011 elements). ( E ) Comparison of recombination rates for non-targeting dual-sgRNA elements between replicates of our K562 growth screen. Non-targeting elements with a growth phenotype (γ>0.05 or γ<−0.05) were excluded (n=973 elements). Recombination rates were weakly correlated between replicates (r=0.30). ( f ) Comparison of recombination rates for all dual-sgRNA elements between replicates of our K562 growth screen (n=20,387 elements). Recombination rates were strongly correlated between replicates (r=0.77). ( G ) Comparison of recombination rates and growth phenotypes for all dual-sgRNA elements in our K562 growth screen (n=20,387 elements). Growth phenotypes are reported as γ. Recombination rates were strongly anticorrelated with growth phenotypes (r=−0.84).
Human Genome Wide Library, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human genome wide library/product/Addgene inc
Average 94 stars, based on 1 article reviews
human genome wide library - by Bioz Stars, 2026-06
94/100 stars
  Buy from Supplier
human foreskin fibroblast p1 library 1 p1  (Genome Systems Inc)
90
Genome Systems Inc human foreskin fibroblast p1 library 1 p1
( A ) Comparison of growth phenotypes for all elements between our pilot single-sgRNA library and Horlbeck et al. data, merged by gene name (n=20,228 elements). Growth phenotypes are reported as γ (log 2 fold-enrichment of T final over T 0 , per doubling) and correlated between experiments (r=0.82). ( B ) Comparison of growth phenotypes for all elements between our pilot dual-sgRNA library and Horlbeck et al. data, merged by gene name (n=20,228 elements). Growth phenotypes are reported as γ and correlated between experiments (r=0.83). ( C ) Comparison of growth phenotypes for all elements between our pilot single- and dual-sgRNA libraries, merged by gene name (n=21,239 with 20,228 targeting elements and 1011 non-targeting elements). Growth phenotypes are reported as γ and correlated between experiments (r=0.86). ( D ) Comparison of true and false-positive rates in single element screens. ‘Positives’ (n=1363 elements) were defined as genes with a K562 <t>CRISPRi</t> growth screen p-value <0.001 and γ<–0.05 , and ‘negatives’ were defined as non-targeting control sgRNA pairs (n=1011 elements). ( E ) Comparison of recombination rates for non-targeting dual-sgRNA elements between replicates of our K562 growth screen. Non-targeting elements with a growth phenotype (γ>0.05 or γ<−0.05) were excluded (n=973 elements). Recombination rates were weakly correlated between replicates (r=0.30). ( f ) Comparison of recombination rates for all dual-sgRNA elements between replicates of our K562 growth screen (n=20,387 elements). Recombination rates were strongly correlated between replicates (r=0.77). ( G ) Comparison of recombination rates and growth phenotypes for all dual-sgRNA elements in our K562 growth screen (n=20,387 elements). Growth phenotypes are reported as γ. Recombination rates were strongly anticorrelated with growth phenotypes (r=−0.84).
Human Foreskin Fibroblast P1 Library 1 P1, supplied by Genome Systems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human foreskin fibroblast p1 library 1 p1/product/Genome Systems Inc
Average 90 stars, based on 1 article reviews
human foreskin fibroblast p1 library 1 p1 - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
pac human genomic library  (Lawrence Livermore National Security LLC)
90
Lawrence Livermore National Security LLC pac human genomic library
( A ) Comparison of growth phenotypes for all elements between our pilot single-sgRNA library and Horlbeck et al. data, merged by gene name (n=20,228 elements). Growth phenotypes are reported as γ (log 2 fold-enrichment of T final over T 0 , per doubling) and correlated between experiments (r=0.82). ( B ) Comparison of growth phenotypes for all elements between our pilot dual-sgRNA library and Horlbeck et al. data, merged by gene name (n=20,228 elements). Growth phenotypes are reported as γ and correlated between experiments (r=0.83). ( C ) Comparison of growth phenotypes for all elements between our pilot single- and dual-sgRNA libraries, merged by gene name (n=21,239 with 20,228 targeting elements and 1011 non-targeting elements). Growth phenotypes are reported as γ and correlated between experiments (r=0.86). ( D ) Comparison of true and false-positive rates in single element screens. ‘Positives’ (n=1363 elements) were defined as genes with a K562 <t>CRISPRi</t> growth screen p-value <0.001 and γ<–0.05 , and ‘negatives’ were defined as non-targeting control sgRNA pairs (n=1011 elements). ( E ) Comparison of recombination rates for non-targeting dual-sgRNA elements between replicates of our K562 growth screen. Non-targeting elements with a growth phenotype (γ>0.05 or γ<−0.05) were excluded (n=973 elements). Recombination rates were weakly correlated between replicates (r=0.30). ( f ) Comparison of recombination rates for all dual-sgRNA elements between replicates of our K562 growth screen (n=20,387 elements). Recombination rates were strongly correlated between replicates (r=0.77). ( G ) Comparison of recombination rates and growth phenotypes for all dual-sgRNA elements in our K562 growth screen (n=20,387 elements). Growth phenotypes are reported as γ. Recombination rates were strongly anticorrelated with growth phenotypes (r=−0.84).
Pac Human Genomic Library, supplied by Lawrence Livermore National Security LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pac human genomic library/product/Lawrence Livermore National Security LLC
Average 90 stars, based on 1 article reviews
pac human genomic library - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
human genomic pac clone  (Genome Systems Inc)
90
Genome Systems Inc human genomic pac clone
( A ) Comparison of growth phenotypes for all elements between our pilot single-sgRNA library and Horlbeck et al. data, merged by gene name (n=20,228 elements). Growth phenotypes are reported as γ (log 2 fold-enrichment of T final over T 0 , per doubling) and correlated between experiments (r=0.82). ( B ) Comparison of growth phenotypes for all elements between our pilot dual-sgRNA library and Horlbeck et al. data, merged by gene name (n=20,228 elements). Growth phenotypes are reported as γ and correlated between experiments (r=0.83). ( C ) Comparison of growth phenotypes for all elements between our pilot single- and dual-sgRNA libraries, merged by gene name (n=21,239 with 20,228 targeting elements and 1011 non-targeting elements). Growth phenotypes are reported as γ and correlated between experiments (r=0.86). ( D ) Comparison of true and false-positive rates in single element screens. ‘Positives’ (n=1363 elements) were defined as genes with a K562 <t>CRISPRi</t> growth screen p-value <0.001 and γ<–0.05 , and ‘negatives’ were defined as non-targeting control sgRNA pairs (n=1011 elements). ( E ) Comparison of recombination rates for non-targeting dual-sgRNA elements between replicates of our K562 growth screen. Non-targeting elements with a growth phenotype (γ>0.05 or γ<−0.05) were excluded (n=973 elements). Recombination rates were weakly correlated between replicates (r=0.30). ( f ) Comparison of recombination rates for all dual-sgRNA elements between replicates of our K562 growth screen (n=20,387 elements). Recombination rates were strongly correlated between replicates (r=0.77). ( G ) Comparison of recombination rates and growth phenotypes for all dual-sgRNA elements in our K562 growth screen (n=20,387 elements). Growth phenotypes are reported as γ. Recombination rates were strongly anticorrelated with growth phenotypes (r=−0.84).
Human Genomic Pac Clone, supplied by Genome Systems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human genomic pac clone/product/Genome Systems Inc
Average 90 stars, based on 1 article reviews
human genomic pac clone - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
human bac libraries  (Genome Systems Inc)
90
Genome Systems Inc human bac libraries
( A ) Comparison of growth phenotypes for all elements between our pilot single-sgRNA library and Horlbeck et al. data, merged by gene name (n=20,228 elements). Growth phenotypes are reported as γ (log 2 fold-enrichment of T final over T 0 , per doubling) and correlated between experiments (r=0.82). ( B ) Comparison of growth phenotypes for all elements between our pilot dual-sgRNA library and Horlbeck et al. data, merged by gene name (n=20,228 elements). Growth phenotypes are reported as γ and correlated between experiments (r=0.83). ( C ) Comparison of growth phenotypes for all elements between our pilot single- and dual-sgRNA libraries, merged by gene name (n=21,239 with 20,228 targeting elements and 1011 non-targeting elements). Growth phenotypes are reported as γ and correlated between experiments (r=0.86). ( D ) Comparison of true and false-positive rates in single element screens. ‘Positives’ (n=1363 elements) were defined as genes with a K562 <t>CRISPRi</t> growth screen p-value <0.001 and γ<–0.05 , and ‘negatives’ were defined as non-targeting control sgRNA pairs (n=1011 elements). ( E ) Comparison of recombination rates for non-targeting dual-sgRNA elements between replicates of our K562 growth screen. Non-targeting elements with a growth phenotype (γ>0.05 or γ<−0.05) were excluded (n=973 elements). Recombination rates were weakly correlated between replicates (r=0.30). ( f ) Comparison of recombination rates for all dual-sgRNA elements between replicates of our K562 growth screen (n=20,387 elements). Recombination rates were strongly correlated between replicates (r=0.77). ( G ) Comparison of recombination rates and growth phenotypes for all dual-sgRNA elements in our K562 growth screen (n=20,387 elements). Growth phenotypes are reported as γ. Recombination rates were strongly anticorrelated with growth phenotypes (r=−0.84).
Human Bac Libraries, supplied by Genome Systems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human bac libraries/product/Genome Systems Inc
Average 90 stars, based on 1 article reviews
human bac libraries - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
p1 genomic library  (DuPont de Nemours)
90
DuPont de Nemours p1 genomic library
Metaphase mapping <t>of</t> <t>NOEY2</t> by fluorescence in situ hybridization. A <t>P1</t> clone containing NOEY2 was mapped to 4′,6-diamidino-2-phenylindole-banded human chromosome 1p31 in normal human lymphocytes. (Inset) Localization of NOEY2 (green) on chromosome 1 counterstained with propidium iodide and 4′,6-diamidino-2-phenylindole compared with 4′,6-diamidino-2-phenylindole-banded chromosome 1.
P1 Genomic Library, supplied by DuPont de Nemours, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p1 genomic library/product/DuPont de Nemours
Average 90 stars, based on 1 article reviews
p1 genomic library - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
genome-scale human transcriptional activation sam library  (GenScript corporation)
90
GenScript corporation genome-scale human transcriptional activation sam library
Metaphase mapping <t>of</t> <t>NOEY2</t> by fluorescence in situ hybridization. A <t>P1</t> clone containing NOEY2 was mapped to 4′,6-diamidino-2-phenylindole-banded human chromosome 1p31 in normal human lymphocytes. (Inset) Localization of NOEY2 (green) on chromosome 1 counterstained with propidium iodide and 4′,6-diamidino-2-phenylindole compared with 4′,6-diamidino-2-phenylindole-banded chromosome 1.
Genome Scale Human Transcriptional Activation Sam Library, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/genome-scale human transcriptional activation sam library/product/GenScript corporation
Average 90 stars, based on 1 article reviews
genome-scale human transcriptional activation sam library - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
p1 phage library of human genomic dna  (Genome Systems Inc)
90
Genome Systems Inc p1 phage library of human genomic dna
Metaphase mapping <t>of</t> <t>NOEY2</t> by fluorescence in situ hybridization. A <t>P1</t> clone containing NOEY2 was mapped to 4′,6-diamidino-2-phenylindole-banded human chromosome 1p31 in normal human lymphocytes. (Inset) Localization of NOEY2 (green) on chromosome 1 counterstained with propidium iodide and 4′,6-diamidino-2-phenylindole compared with 4′,6-diamidino-2-phenylindole-banded chromosome 1.
P1 Phage Library Of Human Genomic Dna, supplied by Genome Systems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p1 phage library of human genomic dna/product/Genome Systems Inc
Average 90 stars, based on 1 article reviews
p1 phage library of human genomic dna - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
pbelobac11 129/svj embryonic stem (es) cell library  (Incyte corporation)
90
Incyte corporation pbelobac11 129/svj embryonic stem (es) cell library
Metaphase mapping <t>of</t> <t>NOEY2</t> by fluorescence in situ hybridization. A <t>P1</t> clone containing NOEY2 was mapped to 4′,6-diamidino-2-phenylindole-banded human chromosome 1p31 in normal human lymphocytes. (Inset) Localization of NOEY2 (green) on chromosome 1 counterstained with propidium iodide and 4′,6-diamidino-2-phenylindole compared with 4′,6-diamidino-2-phenylindole-banded chromosome 1.
Pbelobac11 129/Svj Embryonic Stem (Es) Cell Library, supplied by Incyte corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbelobac11 129/svj embryonic stem (es) cell library/product/Incyte corporation
Average 90 stars, based on 1 article reviews
pbelobac11 129/svj embryonic stem (es) cell library - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier

Image Search Results


( A ) Comparison of growth phenotypes for all elements between our pilot single-sgRNA library and Horlbeck et al. data, merged by gene name (n=20,228 elements). Growth phenotypes are reported as γ (log 2 fold-enrichment of T final over T 0 , per doubling) and correlated between experiments (r=0.82). ( B ) Comparison of growth phenotypes for all elements between our pilot dual-sgRNA library and Horlbeck et al. data, merged by gene name (n=20,228 elements). Growth phenotypes are reported as γ and correlated between experiments (r=0.83). ( C ) Comparison of growth phenotypes for all elements between our pilot single- and dual-sgRNA libraries, merged by gene name (n=21,239 with 20,228 targeting elements and 1011 non-targeting elements). Growth phenotypes are reported as γ and correlated between experiments (r=0.86). ( D ) Comparison of true and false-positive rates in single element screens. ‘Positives’ (n=1363 elements) were defined as genes with a K562 CRISPRi growth screen p-value <0.001 and γ<–0.05 , and ‘negatives’ were defined as non-targeting control sgRNA pairs (n=1011 elements). ( E ) Comparison of recombination rates for non-targeting dual-sgRNA elements between replicates of our K562 growth screen. Non-targeting elements with a growth phenotype (γ>0.05 or γ<−0.05) were excluded (n=973 elements). Recombination rates were weakly correlated between replicates (r=0.30). ( f ) Comparison of recombination rates for all dual-sgRNA elements between replicates of our K562 growth screen (n=20,387 elements). Recombination rates were strongly correlated between replicates (r=0.77). ( G ) Comparison of recombination rates and growth phenotypes for all dual-sgRNA elements in our K562 growth screen (n=20,387 elements). Growth phenotypes are reported as γ. Recombination rates were strongly anticorrelated with growth phenotypes (r=−0.84).

Journal: eLife

Article Title: Maximizing CRISPRi efficacy and accessibility with dual-sgRNA libraries and optimal effectors

doi: 10.7554/eLife.81856

Figure Lengend Snippet: ( A ) Comparison of growth phenotypes for all elements between our pilot single-sgRNA library and Horlbeck et al. data, merged by gene name (n=20,228 elements). Growth phenotypes are reported as γ (log 2 fold-enrichment of T final over T 0 , per doubling) and correlated between experiments (r=0.82). ( B ) Comparison of growth phenotypes for all elements between our pilot dual-sgRNA library and Horlbeck et al. data, merged by gene name (n=20,228 elements). Growth phenotypes are reported as γ and correlated between experiments (r=0.83). ( C ) Comparison of growth phenotypes for all elements between our pilot single- and dual-sgRNA libraries, merged by gene name (n=21,239 with 20,228 targeting elements and 1011 non-targeting elements). Growth phenotypes are reported as γ and correlated between experiments (r=0.86). ( D ) Comparison of true and false-positive rates in single element screens. ‘Positives’ (n=1363 elements) were defined as genes with a K562 CRISPRi growth screen p-value <0.001 and γ<–0.05 , and ‘negatives’ were defined as non-targeting control sgRNA pairs (n=1011 elements). ( E ) Comparison of recombination rates for non-targeting dual-sgRNA elements between replicates of our K562 growth screen. Non-targeting elements with a growth phenotype (γ>0.05 or γ<−0.05) were excluded (n=973 elements). Recombination rates were weakly correlated between replicates (r=0.30). ( f ) Comparison of recombination rates for all dual-sgRNA elements between replicates of our K562 growth screen (n=20,387 elements). Recombination rates were strongly correlated between replicates (r=0.77). ( G ) Comparison of recombination rates and growth phenotypes for all dual-sgRNA elements in our K562 growth screen (n=20,387 elements). Growth phenotypes are reported as γ. Recombination rates were strongly anticorrelated with growth phenotypes (r=−0.84).

Article Snippet: The human CRISPRi dual-sgRNA libraries with IBCs are available from Addgene (hCRISPRi_dual_1_2, Addgene #187246; hCRISPRi_dual_3_4, Addgene #187247; hCRISPRi_dual_5_6, Addgene #187248).

Techniques: Comparison, Control

( A ) Schematics of CRISPRi transcription repressor domains and general lentiviral expression construct used for all CRISPRi effectors. UCOE = ubiquitous chromatin opening element; SFFV = spleen focus-forming virus promoter; P2A = ribosomal skipping sequence; WPRE = woodchuck hepatitis virus post-transcriptional regulatory element. Further information on repressor domains and lentiviral expression constructs can be found in the main text and Materials and methods. ( B ) Experimental design to test effects of stable expression of each CRISPRi effector on growth and transcription in K562 cells. ( C ) Growth defects of effector-expressing cells, measured as the log 2 of the ratio of mCherry-negative (effector-expressing) to mCherry-positive (not effector-expressing) cells in each well normalized to the same ratio on day 0. mCherry levels were measured for 19 days after pooling cells. Data represent mean ± SD from three independent transductions of expression constructs. p-Values are from an unpaired two-tailed t-test comparing D19 values for each sample to the D19 value for the ‘no plasmid’ sample. Average percent growth defect per day is the log 2 D19 value divided by the number of days, multiplied by 100 for a percent value. ( D ) Clustered heatmap of correlation of transcript counts from K562 cells expressing indicated CRISPRi effectors or a GFP control. Correlations across samples were calculated using normalized counts (reads per million) for all genes with mean normalized count >1 and then clustered using the Ward variance minimization algorithm implemented in scipy. r 2 is squared Pearson correlation. Data represent three independent transductions of expression constructs. ( E ) Number of differentially expressed genes ( p <0.05) for cells expressing each effector versus cells expressing GFP only. p -Values were calculated using a Wald test and corrected for multiple hypothesis testing as implemented in DeSeq2. Figure 2—source data 1. p-Values and growth defects depicted in . Figure 2—source data 2. Data depicted in .

Journal: eLife

Article Title: Maximizing CRISPRi efficacy and accessibility with dual-sgRNA libraries and optimal effectors

doi: 10.7554/eLife.81856

Figure Lengend Snippet: ( A ) Schematics of CRISPRi transcription repressor domains and general lentiviral expression construct used for all CRISPRi effectors. UCOE = ubiquitous chromatin opening element; SFFV = spleen focus-forming virus promoter; P2A = ribosomal skipping sequence; WPRE = woodchuck hepatitis virus post-transcriptional regulatory element. Further information on repressor domains and lentiviral expression constructs can be found in the main text and Materials and methods. ( B ) Experimental design to test effects of stable expression of each CRISPRi effector on growth and transcription in K562 cells. ( C ) Growth defects of effector-expressing cells, measured as the log 2 of the ratio of mCherry-negative (effector-expressing) to mCherry-positive (not effector-expressing) cells in each well normalized to the same ratio on day 0. mCherry levels were measured for 19 days after pooling cells. Data represent mean ± SD from three independent transductions of expression constructs. p-Values are from an unpaired two-tailed t-test comparing D19 values for each sample to the D19 value for the ‘no plasmid’ sample. Average percent growth defect per day is the log 2 D19 value divided by the number of days, multiplied by 100 for a percent value. ( D ) Clustered heatmap of correlation of transcript counts from K562 cells expressing indicated CRISPRi effectors or a GFP control. Correlations across samples were calculated using normalized counts (reads per million) for all genes with mean normalized count >1 and then clustered using the Ward variance minimization algorithm implemented in scipy. r 2 is squared Pearson correlation. Data represent three independent transductions of expression constructs. ( E ) Number of differentially expressed genes ( p <0.05) for cells expressing each effector versus cells expressing GFP only. p -Values were calculated using a Wald test and corrected for multiple hypothesis testing as implemented in DeSeq2. Figure 2—source data 1. p-Values and growth defects depicted in . Figure 2—source data 2. Data depicted in .

Article Snippet: The human CRISPRi dual-sgRNA libraries with IBCs are available from Addgene (hCRISPRi_dual_1_2, Addgene #187246; hCRISPRi_dual_3_4, Addgene #187247; hCRISPRi_dual_5_6, Addgene #187248).

Techniques: Expressing, Construct, Virus, Sequencing, Two Tailed Test, Plasmid Preparation, Control

Design of constructs for CRISPR interference (CRISPRi) effector expression.

Journal: eLife

Article Title: Maximizing CRISPRi efficacy and accessibility with dual-sgRNA libraries and optimal effectors

doi: 10.7554/eLife.81856

Figure Lengend Snippet: Design of constructs for CRISPR interference (CRISPRi) effector expression.

Article Snippet: The human CRISPRi dual-sgRNA libraries with IBCs are available from Addgene (hCRISPRi_dual_1_2, Addgene #187246; hCRISPRi_dual_3_4, Addgene #187247; hCRISPRi_dual_5_6, Addgene #187248).

Techniques: Construct, CRISPR, Expressing

( A ) Experimental design to measure knockdown mediated by different CRISPR interference (CRISPRi) effectors by delivering single guide RNAs (sgRNAs) targeting either essential genes or cell surface markers. ( B ) Depletion of K562 cells expressing essential gene-targeting sgRNAs and different CRISPRi effectors, measured as the ratio of mCherry-positive (sgRNA-expressing) to mCherry-negative (not sgRNA-expressing) cells in a given well. mCherry levels were measured for 12 days after transduction, starting on day 3. Data from two replicate transductions. ( C ) Percent knockdown of cell surface markers by different CRISPRi effectors in K562 cells. Cell surface marker levels were measured on day 6 post-transduction by staining with an APC-conjugated antibody. Knockdown was calculated as the ratio of median APC signal in sgRNA-expressing cells and median APC signal in cells expressing a non-targeting control sgRNA after subtraction of background APC signal. Data from two replicate transductions. Cells expressing dCas9 and a strong CD55-targeting sgRNA are represented by a single replicate. ( D ) Distribution of anti-CD151 signal intensity (APC) in individual cells from one representative transduction. Data from second replicate are shown in . Knockdown was quantified as in C as the ratio of the median APC signals. ( E ) Percentage of cells without observable knockdown despite expressing a strong sgRNA, as quantified from the fluorescence distributions.

Journal: eLife

Article Title: Maximizing CRISPRi efficacy and accessibility with dual-sgRNA libraries and optimal effectors

doi: 10.7554/eLife.81856

Figure Lengend Snippet: ( A ) Experimental design to measure knockdown mediated by different CRISPR interference (CRISPRi) effectors by delivering single guide RNAs (sgRNAs) targeting either essential genes or cell surface markers. ( B ) Depletion of K562 cells expressing essential gene-targeting sgRNAs and different CRISPRi effectors, measured as the ratio of mCherry-positive (sgRNA-expressing) to mCherry-negative (not sgRNA-expressing) cells in a given well. mCherry levels were measured for 12 days after transduction, starting on day 3. Data from two replicate transductions. ( C ) Percent knockdown of cell surface markers by different CRISPRi effectors in K562 cells. Cell surface marker levels were measured on day 6 post-transduction by staining with an APC-conjugated antibody. Knockdown was calculated as the ratio of median APC signal in sgRNA-expressing cells and median APC signal in cells expressing a non-targeting control sgRNA after subtraction of background APC signal. Data from two replicate transductions. Cells expressing dCas9 and a strong CD55-targeting sgRNA are represented by a single replicate. ( D ) Distribution of anti-CD151 signal intensity (APC) in individual cells from one representative transduction. Data from second replicate are shown in . Knockdown was quantified as in C as the ratio of the median APC signals. ( E ) Percentage of cells without observable knockdown despite expressing a strong sgRNA, as quantified from the fluorescence distributions.

Article Snippet: The human CRISPRi dual-sgRNA libraries with IBCs are available from Addgene (hCRISPRi_dual_1_2, Addgene #187246; hCRISPRi_dual_3_4, Addgene #187247; hCRISPRi_dual_5_6, Addgene #187248).

Techniques: Knockdown, CRISPR, Expressing, Transduction, Marker, Staining, Control, Fluorescence

( A ) Depletion of K562 cells expressing essential gene-targeting single guide RNAs (sgRNAs) and different CRISPRi effectors, measured as the ratio of mCherry-positive (sgRNA-expressing) to mCherry-negative (not sgRNA-expressing) cells in a given well, as in . mCherry levels were measured for 12 days after transduction, starting on day 3. Data from two replicate transductions. ( B ) Distribution of anti-CD151 signal intensity (APC) in K562 cells expressing indicated CRISPRi effectors from second replicate transduction. Knockdown was quantified as in . ( C ) Distribution of anti-CD81 signal intensity (APC) in K562 cells expressing indicated CRISPRi effectors from two replicate transductions. Knockdown was quantified as in . ( D ) Distribution of anti-CD55 signal intensity (APC) in K562 cells expressing indicated CRISPRi effectors from two replicate transductions. Cells expressing dCas9 and the CD55-targeting sgRNA are represented by a single replicate. Knockdown was quantified as in .

Journal: eLife

Article Title: Maximizing CRISPRi efficacy and accessibility with dual-sgRNA libraries and optimal effectors

doi: 10.7554/eLife.81856

Figure Lengend Snippet: ( A ) Depletion of K562 cells expressing essential gene-targeting single guide RNAs (sgRNAs) and different CRISPRi effectors, measured as the ratio of mCherry-positive (sgRNA-expressing) to mCherry-negative (not sgRNA-expressing) cells in a given well, as in . mCherry levels were measured for 12 days after transduction, starting on day 3. Data from two replicate transductions. ( B ) Distribution of anti-CD151 signal intensity (APC) in K562 cells expressing indicated CRISPRi effectors from second replicate transduction. Knockdown was quantified as in . ( C ) Distribution of anti-CD81 signal intensity (APC) in K562 cells expressing indicated CRISPRi effectors from two replicate transductions. Knockdown was quantified as in . ( D ) Distribution of anti-CD55 signal intensity (APC) in K562 cells expressing indicated CRISPRi effectors from two replicate transductions. Cells expressing dCas9 and the CD55-targeting sgRNA are represented by a single replicate. Knockdown was quantified as in .

Article Snippet: The human CRISPRi dual-sgRNA libraries with IBCs are available from Addgene (hCRISPRi_dual_1_2, Addgene #187246; hCRISPRi_dual_3_4, Addgene #187247; hCRISPRi_dual_5_6, Addgene #187248).

Techniques: Expressing, Transduction, Knockdown

( A ) Distribution of anti-B2M signal intensity (APC) in individual RPE1 (left) and Jurkat (right) cells expressing indicated CRISPR interference (CRISPRi) effectors and single guide RNAs (sgRNAs). Knockdown was calculated as the ratio of median APC signal in transduced (sgRNA-expressing) cells and median APC signal in non-transduced cells in the same well, after subtraction of background APC signal. ( B ) Depletion of indicated cell surface markers in HepG2 (top), HuTu-80 (middle), and HT29 (bottom) cells expressing Zim3-dCas9. Cell surface marker levels were measured 6–14 days post-transduction by staining with APC-conjugated antibodies. Knockdown was calculated as the ratio of median APC signal in sgRNA-expressing cells and median APC signal in cells expressing a non-targeting control sgRNA after subtraction of background APC signal. ( C ) Distribution of anti-B2M signal intensity (APC) in individual K562 cells expressing indicated CRISPRi effectors and sgRNAs. The Zim3-dCas9 (Hygro) cell line was generated by transduction followed by hygromycin selection and does not express a fluorescent protein. Knockdown was calculated as in A .

Journal: eLife

Article Title: Maximizing CRISPRi efficacy and accessibility with dual-sgRNA libraries and optimal effectors

doi: 10.7554/eLife.81856

Figure Lengend Snippet: ( A ) Distribution of anti-B2M signal intensity (APC) in individual RPE1 (left) and Jurkat (right) cells expressing indicated CRISPR interference (CRISPRi) effectors and single guide RNAs (sgRNAs). Knockdown was calculated as the ratio of median APC signal in transduced (sgRNA-expressing) cells and median APC signal in non-transduced cells in the same well, after subtraction of background APC signal. ( B ) Depletion of indicated cell surface markers in HepG2 (top), HuTu-80 (middle), and HT29 (bottom) cells expressing Zim3-dCas9. Cell surface marker levels were measured 6–14 days post-transduction by staining with APC-conjugated antibodies. Knockdown was calculated as the ratio of median APC signal in sgRNA-expressing cells and median APC signal in cells expressing a non-targeting control sgRNA after subtraction of background APC signal. ( C ) Distribution of anti-B2M signal intensity (APC) in individual K562 cells expressing indicated CRISPRi effectors and sgRNAs. The Zim3-dCas9 (Hygro) cell line was generated by transduction followed by hygromycin selection and does not express a fluorescent protein. Knockdown was calculated as in A .

Article Snippet: The human CRISPRi dual-sgRNA libraries with IBCs are available from Addgene (hCRISPRi_dual_1_2, Addgene #187246; hCRISPRi_dual_3_4, Addgene #187247; hCRISPRi_dual_5_6, Addgene #187248).

Techniques: Expressing, CRISPR, Knockdown, Marker, Transduction, Staining, Control, Generated, Selection

Journal: eLife

Article Title: Maximizing CRISPRi efficacy and accessibility with dual-sgRNA libraries and optimal effectors

doi: 10.7554/eLife.81856

Figure Lengend Snippet:

Article Snippet: The human CRISPRi dual-sgRNA libraries with IBCs are available from Addgene (hCRISPRi_dual_1_2, Addgene #187246; hCRISPRi_dual_3_4, Addgene #187247; hCRISPRi_dual_5_6, Addgene #187248).

Techniques: Stable Transfection, Marker, Flow Cytometry, Recombinant, Plasmid Preparation, Sequencing, Expressing, Purification, Amplification, Transfection, Software, Genome Wide

Metaphase mapping of NOEY2 by fluorescence in situ hybridization. A P1 clone containing NOEY2 was mapped to 4′,6-diamidino-2-phenylindole-banded human chromosome 1p31 in normal human lymphocytes. (Inset) Localization of NOEY2 (green) on chromosome 1 counterstained with propidium iodide and 4′,6-diamidino-2-phenylindole compared with 4′,6-diamidino-2-phenylindole-banded chromosome 1.

Journal:

Article Title: NOEY2 (ARHI), an imprinted putative tumor suppressor gene in ovarian and breast carcinomas

doi:

Figure Lengend Snippet: Metaphase mapping of NOEY2 by fluorescence in situ hybridization. A P1 clone containing NOEY2 was mapped to 4′,6-diamidino-2-phenylindole-banded human chromosome 1p31 in normal human lymphocytes. (Inset) Localization of NOEY2 (green) on chromosome 1 counterstained with propidium iodide and 4′,6-diamidino-2-phenylindole compared with 4′,6-diamidino-2-phenylindole-banded chromosome 1.

Article Snippet: The NOEY2 gene was mapped to chromosome 1p31 by screening a P1 genomic library (DuPont) with two sets of primers that encompass the entire coding and 3′ untranslated region.

Techniques: Fluorescence, In Situ Hybridization